Journal: Pharmaceuticals

Article Title: Fluorochrome Selection for Imaging Intraoperative Ovarian Cancer Probes

doi: 10.3390/ph15060668

Figure Lengend Snippet: Organ biodistribution and intraoperative NIR FLI; ( A ) “Ex vivo” NIR FLI 6 h after 500 µM injection of RR11 and 23 ( MSA14 ) was performed to assess the compound accumulation in tumors and organs. ( B ) Representative intraoperative near-infrared (NIR), color and pseudo-colored fluorescent signal merge images of one 23 ( MSA14 ) injected subcutaneously engrafted OVCAR-3 mouse in vivo and ex vivo.

Article Snippet: A second intraoperative compatible imaging system (FLARE ® , Curadel LLC, Nattick, MA, USA) was used to assess the clinical utility of 23 ( MSA14 ).

Techniques: Ex Vivo, Injection, In Vivo

Journal: Pharmaceuticals

Article Title: Fluorochrome Selection for Imaging Intraoperative Ovarian Cancer Probes

doi: 10.3390/ph15060668

Figure Lengend Snippet: Biodistribution of RR11 and 23 ( MSA14 ) in SKOV-3 s.c. models. RR11 ( A ) and 23 ( MSA14 ) ( B ) were intravenously injected into n = 2 and n = 4 mice, respectively, and imaged at 2, 6, and 24 h with a dose of 500 and 100 µM. The fluorescence signal in the tumors (*), liver (#), limb (†), spine (‡) and background in the flank muscle (yellow square), is presented in one representative mouse. All animals were euthanized when indicated and “ex vivo” NIR FLI was performed. ( C ) 23 (MSA14) injected SKOV-3 mice were additionally imaged with the intraoperative imaging system “in vivo” and “ex vivo”. NIR exposure times are indicated in brackets.

Article Snippet: A second intraoperative compatible imaging system (FLARE ® , Curadel LLC, Nattick, MA, USA) was used to assess the clinical utility of 23 ( MSA14 ).

Techniques: Injection, Fluorescence, Ex Vivo, Imaging, In Vivo

Journal: Journal of neurosurgery

Article Title: Folate receptor overexpression can be visualized in real time during pituitary adenoma endoscopic transsphenoidal surgery with near-infrared imaging

doi: 10.3171/2017.2.JNS163191

Figure Lengend Snippet: Visionsense equipment setup for NIR imaging. Upper: The excitation laser for NIR imaging is attached to a 4-mm endoscope. Copyright Visionsense. Published with permission. Lower: Images are displayed on the leftmost screen.

Article Snippet: Visionsense equipment setup for NIR imaging.

Techniques: Imaging

Journal: Journal of neurosurgery

Article Title: Folate receptor overexpression can be visualized in real time during pituitary adenoma endoscopic transsphenoidal surgery with near-infrared imaging

doi: 10.3171/2017.2.JNS163191

Figure Lengend Snippet: Case 7. This patient had an NF adenoma, high FRα expression on final pathological analysis, and intraoperative NIR fluorescence. A and B: Preoperative sagittal (A) and coronal (B) MR images without contrast showing a 17-mm mass in the sella. C and D: Intraoperative endoscopic imaging with the dura open shown with white light only (C) and the NIR signal superimposed on white light (D). The MOCRs can be seen clearly and are used as a measure of distance from the endoscope to the target. E and F: Intraoperative endoscopic image of the sella after resection of the gross tumor. The residual tumor was identified on the right, both with white light only (E) and the NIR signal superimposed on the white light (F). This fluorescent specimen was confirmed on the final pathological analysis to be consistent with pituitary adenoma. G and H: Intraoperative endoscopic image of sella contents after resection of the residual neoplasm with white light only (G) and NIR superimposed on white light (H). No obvious fluorescence is identified. I: Example of SBR and MOCR calculation. Five points are chosen in the gross tumor itself, with the Visionsense camera calculating NIR signal for the points (75 + 74 + 66 + 61 + 57)/5 = 67 ± 7.9. Five points are chosen in the surrounding bone close to the tumor but outside of the obvious specimen (22 + 29 + 34 + 19 + 17)/5 = 24 ± 7.1. The SBR is thus 67/24 = 2.8. The black triangles denote the locations of the MOCRs, spanning roughly 50% of the field, that were used to approximate the endoscope distance.

Article Snippet: Visionsense equipment setup for NIR imaging.

Techniques: Expressing, Fluorescence, Imaging

Journal: Cancer Medicine

Article Title: Trastuzumab‐based near‐infrared photoimmunotherapy in xenograft mouse of breast cancer

doi: 10.1002/cam4.5302

Figure Lengend Snippet: Evaluation of NIR‐PIT effects in BT‐474 tumor model. (A) The regimen of NIR‐PIT in vivo. Fluorescence images were obtained at each time point as indicated. (B) Time‐course imaging of in vivo Tra‐IR700 fluorescence in BT‐474 tumor‐bearing mice in response to NIR‐PIT. Tumors treated with NIR‐PIT showed decreased Tra‐IR700 fluorescence just after NIR light irradiation. Upper‐left panel uses same data as shown in Figure (right‐middle panel). (C) Intensity ratio of Tra‐IR700 fluorescence before and after NIR‐PIT treatment. The NIR‐PIT‐treated group showed significantly decreased Tra‐IR700 fluorescence compared with the Tra‐IR700 only group ( n = 3–10; Aspin–Welch's t ‐test; ** p < 0.05). (D) Histological images of resected BT‐474 tumor 1 day after NIR‐PIT. In the NIR‐PIT group, tumor cells showed diffuse cellular necrosis and micro‐hemorrhage. Black scale bars = 1 mm. White scale bars = 50 μm. (E) Quantification of damaged areas of tumor tissue by NIR‐PIT. The damaged areas of tumor tissue were scored by percentage (%) in histological images. In the NIR‐PIT group, the tumor tissue damage was significantly increased compared with the NIR‐PIT‐untreated group ( n = 5–10; Aspin–Welch's t ‐test: ** p < 0.05). (F) NADH‐TR‐stained images of BT‐474 tumor sections 1 day after NIR‐PIT. In the NIR‐PIT group, NADH staining was lacking. Black scale bars = 1 mm. White scale bars = 50 μm.

Article Snippet: Macroscopic images of mice were captured using an in‐house NIR fluorescence imaging system consisting of excitation light by LEDs at a peak of 660 nm (SMBB660‐1100‐02; Ushio Opto Semiconductors) and a monochrome camera (GS3‐U3‐15S5M‐C, Point Grey Research) equipped with a 692‐nm long‐pass filter (FF01‐692/LP‐25‐D; Semrock).

Techniques: In Vivo, Fluorescence, Imaging, Irradiation, Staining